Isolation and properties of the leukocytosis- and lymphocytosis- promoting factor of Bordetella pertussis
نویسندگان
چکیده
The leukocytosis- and lymphocytosis-promoting factor (LPF) of Bordetella pertussis has been isolated to near homogeneity by physical, chemical, and electron microscopical criteria. LPF contains 14.5% nitrogen and is lipid and carbohydrate free. It is apparently composed of four polypeptide subunits. LPF caused leukocytosis and lymphocytosis in "nude" as well as in normal mice. In addition, purified LPF also induced histamine sensitization and hypoglycemia and refractoriness to the hyperglycemic effect of epinephrine. A monospecific LPF antiserum blocked these reactions as well as leukocytosis and lymphocytosis. LPF is clearly distinct from the hemagglutinating pili of B. pertussis.
منابع مشابه
Lymphocytosis-promoting factor of Bordetella pertussis alters mononuclear phagocyte circulation and response to inflammation.
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The leukocytosis-promoting factor (LPF), purified from materials extracted from agar in which Bordetella pertussis was grown, caused leukocytosis and an increased sensitivity to histamine when as little as 0.04 Ag of protein was given intravenously to mice. LPF was adsorbed onto erythrocytes, causing hemagglutination. As little as 0.03 ,g of LPF protein agglutinated chicken erythrocytes in 0.05...
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ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 143 شماره
صفحات -
تاریخ انتشار 1976